At a glance

Also known as

Ethyl alcohol; alcohol; EtOH

Why get tested?

To determine if a person has consumed ethanol (alcohol) and to measure the amount of ethanol present

When to get tested?

When a patient has symptoms that suggest ethanol toxicity or when a person is suspected of violating drinking-related laws or as part of a drug testing panel

Sample required?

Ethanol may be determined from a blood sample, a urine sample, a saliva (oral fluid) sample or a breath sample. Some samples, such as breath samples, are usually analysed immediately on-site, rather than being sent to a laboratory.

Test preparation needed?


What is being tested?

This test measures the amount of ethanol in the blood, urine, breath or saliva (oral fluid). Ethanol (also called ethyl alcohol or alcohol) has been consumed by civilisations throughout the world for thousands of years. Small amounts of ethanol can cause euphoria, relaxation and decreased inhibition. Moderate amounts can cause impaired judgment and decreased motor skills; large amounts in a relatively short period of time can cause acute ethanol toxicity with disorientation, depressed breathing, coma and even death. Chronic ingestion of large quantities of ethanol can lead to alcoholism, permanent liver damage and many other health effects.

When ethanol is consumed, it is absorbed by the gastrointestinal tract and carried throughout the body in the bloodstream. Small amounts of ethanol are excreted in the urine or exhaled from the lungs, but most is metabolised by the liver. The liver breaks down ethanol using enzymes, that oxidise the ethanol first to acetaldehyde, then to acetate, and then finally to carbon dioxide and water. The liver can process about one standard drink an hour, although this varies from person to person – with one standard drink being defined as the amount of drink containing 10g of alcohol (ethanol). A person who drinks more than 1 drink an hour will have a build up of ethanol in their blood stream.

How is the sample collected for testing?

A blood sample is obtained by inserting a needle into a vein in the arm. A breath sample is collected by blowing or speaking into a device. Urine samples are collected in plastic containers. Saliva (oral fluid) samples are often collected from the mouth using a swab.

Is any test preparation needed to ensure the quality of the sample?

No test preparation is needed.

The Test

How is it used?

The ethanol test is used for both medical and legal purposes.

Medical testing
The goal of medical testing is to identify the presence of ethanol in order to effectively treat the patient's symptoms. For medical purposes blood and sometimes urine or breath tests are used to detect the presence of ethanol and to measure its concentration. One or more of these tests may be ordered when a patient presents to a hospital emergency department with symptoms suggesting ethanol toxicity. Symptoms may include confusion, staggering, vomiting, lethargy and unconsciousness.

Occasionally, other tests for by-products of ethanol breakdown in the body may be done, such as urine testing for ethyl glucuronide. The patient may also be tested for other types of drugs and poisons and for other medical conditions.

Legal testing
The goal of legal testing is to identify the presence of ethanol and to evaluate its presence in the context of a variety of different laws. Legal testing must be done by specially trained people and the handling of the sample is carefully recorded in a procedure known as chain-of-custody. Testing may be ordered to determine whether a motor vehicle driver has a blood ethanol concentration that is over the legal limit; whether someone ordered by a court not to drink ethanol has done so; and to determine whether ethanol consumption has contributed to an accident. Post-mortem ethanol testing may be done to determine whether ethanol contributed to a person’s death. Legal ethanol testing may also be performed randomly or ‘with cause’ as part of an employer's drug testing program to determine whether an employee has ethanol in their system.

Samples tested for legal purposes may include blood or breath. Breath testing is the most common test performed on drivers. It uses the tail-end sample of breath from deep in the lungs and uses a conversion factor to estimate the amount of ethanol in the blood. Blood ethanol testing may be ordered to confirm or refute findings, and/or ordered as an alternative to breath testing. A random urine sample is sometimes ordered to monitor people for the presence of ethanol. Saliva (oral fluid) ethanol testing is becoming available as an alternative screening test.

In some cases people may be required to undergo regular testing to show they are limiting their ethanol intake on a long-term basis. This is done by checking the level of a naturally-occurring substance in the blood known as CDT (carbohydrate deficient transferrin), which may be increased for weeks after ethanol intake.

When is it requested?

Medical ethanol testing is ordered to help doctors treating a patient who has symptoms that suggest intoxication with ethanol.

Legal ethanol testing may be ordered whenever there is reason to suspect that a person has not followed a drinking-related law, and whenever there has been an accident and/or unexpected death to determine whether ethanol played a role. Employment ethanol testing may be performed randomly and when the employer suspects that an employee has ethanol in their system while on the job.

What does the test result mean?

For medical testing, the detection of ethanol in a sample indicates that the person's symptoms may be due to ethanol and the concentration present can give an indication of how severe the ethanol toxicity is. For legal testing, the person's result is compared to legal allowable limits.

About Reference Intervals

Is there anything else I should know?

The ethanol level measured in a breath sample gives a good estimate of a person's blood ethanol, but a positive breath test result may be checked by performing a blood test.

Breath sample concentrations can be affected by ethanol present in the mouth from consumption within the last few minutes, including the use of substances that may contain ethanol such as mouthwash and cough syrup.

Common Questions

Are medical ethanol results released for legal purposes?

Rules vary from state to state. In most cases, medical and legal results are evaluated separately.

Can I choose which sample will be collected?

In most cases, there is a primary type of sample that is collected, but another alternative is often available for confirmation and/or to refute findings.

Does everyone metabolise alcohol at the same rate?

A general rule of thumb is one standard drink an hour, but there is individual variation due to differences in factors such as race, gender and body weight.

Last Review Date: September 21, 2022

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