Chemical tests 
Albumin - may be similar to the plasma albumin concentration or significantly lower. A Serum Ascites-Albumin Gradient (SAAG) is calculated as the difference between the serum albumin concentration and the ascitic fluid albumin concentration.

Tests that may be performed in addition to albumin include:
Glucose - typically about the same as blood glucose levels but may be lower with infection.
Amylase - increased with pancreatic disease.
Total protein - may be requested instead of albumin, but cannot be used in the SAAG calculation.

Microscopic examination - may be performed if infection or cancer is suspected. Normal peritoneal fluid has small numbers of white blood cells (WBCs) but no red blood cells (RBCs) or microorganisms.
Total cell counts - WBCs and RBCs in the sample are enumerated. Increased WBCs may be seen with infections and malignant conditions.
WBC differential - determination of percentages of different types of WBCs. An increased number of neutrophils may be seen with bacterial infections.
Cytology - a cytocentrifuged sample is treated with a special stain and examined under a microscope for abnormal cells and for white cell differentiation. The differential can help determine whether the cells are the result of an infection or the presence of a tumour.

Infectious disease tests - tests may be performed to look for microorganisms if infection is suspected.
Gram stain - for direct observation of bacteria or fungi under a microscope. There should be no organisms in peritoneal fluid.
Bacterial culture and susceptibility testing - ordered to detect any microorganisms, which will grow in the culture. If bacteria are present, susceptibility testing can be performed to guide antimicrobial therapy.
Other peritoneal fluid tests for infectious diseases that are less commonly ordered include tests for viruses, mycobacteria (Acid fast bacilli culture) or parasites.

Peritoneal fluid analysis may be ordered when a doctor suspects that a patient has a condition or disease that is causing peritonitis and/or ascites. 

The common cause of a high SAAG value is cirrhosis of the liver but congestive heart failure, cancer metastatic to the liver or carcinoma of the liver itself (hepatoma) may also cause an elevated SAAG.

A low SAAG value may be found in peritonitis from connective tissue disorders, bowel perforation or infarction, in tuberculous peritonitis, pancreatitis and peritoneal malignancy.

Infectious diseases are caused by viruses, bacteria, or fungi. Infections may originate in the peritoneum, be due to a rupture of the appendix, perforation of the intestines or the abdominal wall, contamination during surgery, or may spread to the peritoneum from other places in the body.

Plasma glucose, albumin and total protein may be ordered to compare concentrations with those in the peritoneal fluid. If a doctor suspects that a patient may have a systemic infection, then a blood culture may be ordered in addition to the peritoneal fluid analysis.

Paracentesis is the removal of peritoneal fluid from the abdominal cavity with a needle, tubing, and a container that may have a vacuum. The patient is positioned lying down with the head of the bed raised. A local anaesthetic is applied and then the doctor inserts the needle into the abdominal cavity and the fluid is removed.

Yes. Sometimes it will be performed to drain excess peritoneal fluid - to relieve pressure in the abdomen. The volume of fluid removed may be large - sometimes as much as four litres or more. This may need to be repeated periodically with some diseases.